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. 2002 Dec;10(6):1269-81.
doi: 10.1016/s1097-2765(02)00728-1.

Mechanism of recruitment of WASP to the immunological synapse and of its activation following TCR ligation

Yoji Sasahara  1 , Rima Rachid, Michael J Byrne, Miguel A de la Fuente, Robert T Abraham, Narayanaswamy Ramesh, Raif S Geha
Affiliations
Free article

Mechanism of recruitment of WASP to the immunological synapse and of its activation following TCR ligation

Yoji Sasahara et al. Mol Cell. 2002 Dec.
Free article

Abstract

F-actin polymerization following engagement of the T cell receptor (TCR) is dependent on WASP and is critical for T cell activation. The link between TCR and WASP is not fully understood. In resting cells, WASP exists in a complex with WIP, which inhibits its activation by Cdc42. We show that the adaptor protein CrkL binds directly to WIP. Further, TCR ligation results in the formation of a ZAP-70-CrkL-WIP-WASP complex, which is recruited to lipid rafts and the immunological synapse. TCR engagement also causes PKCtheta-dependent phosphorylation of WIP, causing the disengagement of WASP from the WIP-WASP complex, thereby releasing it from WIP inhibition. These results suggest that the ZAP-70-CrkL-WIP pathway and PKCtheta link TCR to WASP activation.

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