doi: 10.1073/pnas.73.12.4415.
Isolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase
- PMID: 12506
- PMCID: PMC431479
- DOI: 10.1073/pnas.73.12.4415
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Isolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase
Proc Natl Acad Sci U S A.
1976 Dec.
Abstract
Pyruvate, phosphate dikinase (EC 2-7-9-1) catalyzes formation of phosphoenolpyruvate, AMP, and inorganic pyrophosphate from pyruvate, ATP, and orthophosphate. A pyrophosphoryl and phosphoryl form of the enzyme is involved in this transfer. The [32P]phosphoryl form of pyruvate, phosphate dikinase was prepared with enzyme isolated from Bacteroides symbiosus. The [32P]phosphoryl enzyme was found to have properties corresponding to a phosphoramidate linkage and this was confirmed by isolation of 3-[32P]phosphohistidine from alkaline hydrolysates of the enzyme. The histidyl residue is considered to be the pyrophosphoryl- and phosphoryl-carrier between the three substrate sites of this enzyme.
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