Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Dec;73(12):4415-9.
doi: 10.1073/pnas.73.12.4415.

Isolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase

A M Spronk, H Yoshida, H G Wood

Isolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase

A M Spronk et al. Proc Natl Acad Sci U S A. 1976 Dec.

Abstract

Pyruvate, phosphate dikinase (EC 2-7-9-1) catalyzes formation of phosphoenolpyruvate, AMP, and inorganic pyrophosphate from pyruvate, ATP, and orthophosphate. A pyrophosphoryl and phosphoryl form of the enzyme is involved in this transfer. The [32P]phosphoryl form of pyruvate, phosphate dikinase was prepared with enzyme isolated from Bacteroides symbiosus. The [32P]phosphoryl enzyme was found to have properties corresponding to a phosphoramidate linkage and this was confirmed by isolation of 3-[32P]phosphohistidine from alkaline hydrolysates of the enzyme. The histidyl residue is considered to be the pyrophosphoryl- and phosphoryl-carrier between the three substrate sites of this enzyme.

PubMed Disclaimer

References

    1. J Biol Chem. 1973 Feb 10;248(3):941-56 - PubMed
    1. FEBS Lett. 1972 Feb 1;20(2):167-170 - PubMed
    1. Biochemistry. 1975 Jul 15;14(14):3213-9 - PubMed
    1. Biochemistry. 1971 Jul 6;10(14):2707-10 - PubMed
    1. Eur J Biochem. 1969 Jul;9(4):451-5 - PubMed

Publication types

LinkOut - more resources