Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Jan;3(1):219-29.
doi: 10.1093/nar/3.1.219.

Thermostable polynucleotide phosphorylases from Bacillus stearothermophilus and Thermus aquaticus

J N Wood, D W Hutchinson
Free PMC article

Thermostable polynucleotide phosphorylases from Bacillus stearothermophilus and Thermus aquaticus

J N Wood et al. Nucleic Acids Res. 1976 Jan.
Free PMC article

Abstract

Polynucleotide phosphorylase from Bacillus stearothermophilus has been purified to homogeneity. Polyacrylamide gel electrophoresis run under denaturing conditions indicates that the enzyme is a tetramer with subunits of apparent molecular weight 51,000 daltons. A partial purification of polynucleotide phosphorylase from Thermus aquaticus has also been effected. The two enzymes show similar catalytic properties, which differ little from those of mesophilic polynucleotide phosphorylases. The use of thermostable polynucleotide phosphorylases for in vitro nucleic acid synthesis is discussed.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1975 Feb;2(2):149-64 - PubMed
    1. J Biol Chem. 1972 May 25;247(10):3242-51 - PubMed
    1. Biochemistry. 1972 Sep 12;11(19):3544-58 - PubMed
    1. Methods Enzymol. 1972;26:3-27 - PubMed
    1. Biochem Biophys Res Commun. 1969 Mar 10;34(5):640-5 - PubMed

MeSH terms

Substances

LinkOut - more resources