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. 2003 Jan;9(1):125-8.
doi: 10.3748/wjg.v9.i1.125.

N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat

Resat Ozaras  1 , Veysel Tahan, Seval Aydin, Hafize Uzun, Safiye Kaya, Hakan Senturk
Affiliations

N-acetylcysteine attenuates alcohol-induced oxidative stress in the rat

Resat Ozaras et al. World J Gastroenterol. 2003 Jan.

Abstract

Aim: There is increasing evidence that alcohol-induced liver damage may be associated with increased oxidative stress. We aimed to investigate free-radical scavenger effect of n-acetylcysteine in rats intragastrically fed with ethanol.

Methods: Twenty-four rats divided into three groups were fed with ethanol (6 g/kg/day, Group 1), ethanol and n-acetylcysteine (1 g/kg, Group 2), or isocaloric dextrose (control group, Group 3) for 4 weeks. Then animals were sacrificed under ether anesthesia, intracardiac blood and liver tissues were obtained. Measurements were performed both in serum and in homogenized liver tissues. Malondialdehyde (MDA) level was measured by TBARS method. Glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels were studied by commercial kits. Kruskal-Wallis test was used for statistical analysis.

Results: ALT and AST in Group 1 (154 U/L and 302 U/L, respectively) were higher than those in Group 2 (94 U/L and 155 U/L) and Group 3 (99 U/L and 168 U/L) (P=0.001 for both). Serum and tissue levels of MDA in Group 1 (1.84 nmol/mL and 96 nmol/100 mg-protein) were higher than Group 2 (0.91 nmol/mL and 64 nmol/100 mg-protein) and Group 3 (0.94 nmol/mL and 49 nmol/100 mg-protein) (P<0.001 for both). On the other hand, serum GSH-Px level in Group 1 (8.21 U/g-Hb) was lower than Group 2 (16 U/g-Hb) and Group 3 (16 U/g-Hb) (P<0.001). Serum and liver tissue levels of SOD in Group 1 (11 U/mL and 26 U/100 mg-protein) were lower than Group 2 (18 U/mL and 60 U/100 mg-protein) and Group 3 (20 U/mL and 60 U/100 mg-protein) (P<0.001 for both).

Conclusion: This study demonstrated that ethanol-induced liver damage is associated with oxidative stress, and co-administration of n-acetylcysteine attenuates this damage effectively in rat model.

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Figures

Figure 1
Figure 1
Serum AST and ALT levels (U/L). Alc: Alcohol, NAC: N-acetylcysteine, bP = 0.001 vs control and Alc + NAC group.
Figure 2
Figure 2
Serum (U/L) and tissue (U/g-protein) GGT levels. Alc: Alcohol, NAC: N-acetylcysteine, bP < 0.001 vs control and Alc + NAC group.
Figure 3
Figure 3
Serum (U/L) and Tissue (U/g-protein) ALP levels Alc: Alcohol, NAC: N-acetylcysteine, bP < 0.001 vs control and Alc + NAC group.
Figure 4
Figure 4
Erythrocyte glutathione levels (U/g-Hb). Alc: Alcohol, NAC: N-acetylcysteine, bP < 0.01 vs control and Alc + NAC group.
Figure 5
Figure 5
Blood glutathione peroxidase levels (U/g-Hb). Alc: Alcohol, NAC: N-acetylcysteine, aP < 0.001 vs control and Alc + NAC group.
Figure 6
Figure 6
Serum (U/mL) and tissue (U/100 mg-protein) superoxide dismutase (SOD) levels. Alc: Alcohol, NAC: N-acetylcysteine, bP < 0.001 vs control and Alc + NAC group.
Figure 7
Figure 7
Serum MDA level (nmol/mL). Alc: Alcohol, NAC: N-acetylcysteine, bP < 0.001 vs control and Alc + NAC group.
Figure 8
Figure 8
Tissue MDA level (nmol/100 mg-protein). Alc: Alcohol, NAC: N-acetylcysteine, bP < 0.001 vs control and Alc + NAC group.
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References

    1. Bruck R, Aeed H, Shirin H, Matas Z, Zaidel L, Avni Y, Halpern Z. The hydroxyl radical scavengers dimethylsulfoxide and dimethylthiourea protect rats against thioacetamide-induced fulminant hepatic failure. J Hepatol. 1999;31:27–38. - PubMed
    1. Bacon BR, Tavill AS, Brittenham GM, Park CH, Recknagel RO. Hepatic lipid peroxidation in vivo in rats with chronic iron overload. J Clin Invest. 1983;71:429–439. - PMC - PubMed
    1. Kyle ME, Miccadei S, Nakae D, Farber JL. Superoxide dismutase and catalase protect cultured hepatocytes from the cytotoxicity of acetaminophen. Biochem Biophys Res Commun. 1987;149:889–896. - PubMed
    1. Shaw S, Jayatilleke E, Ross WA, Gordon ER, Leiber CS. Ethanol-induced lipid peroxidation: potentiation by long-term alcohol feeding and attenuation by methionine. J Lab Clin Med. 1981;98:417–424. - PubMed
    1. Dai Y, Rashba-Step J, Cederbaum AI. Stable expression of human cytochrome P4502E1 in HepG2 cells: characterization of catalytic activities and production of reactive oxygen intermediates. Biochemistry. 1993;32:6928–6937. - PubMed

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