Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Jan;9(1):137-40.
doi: 10.3748/wjg.v9.i1.137.

Influence of intrauterine injection of rat fetal hepatocytes on rejection of rat liver transplantation

Yan-Ling Yang  1 , Ke-Feng Dou, Kai-Zong Li
Affiliations

Influence of intrauterine injection of rat fetal hepatocytes on rejection of rat liver transplantation

Yan-Ling Yang et al. World J Gastroenterol. 2003 Jan.

Abstract

Aim: To investigate the influence of immune tolerance induced by intrauterine exposure to fetal hepatocytes on liver transplantation in the adult rat.

Methods: LOU/CN rat fetal hepatocytes were injected into the fetuses of pregnant CHN rats (14-16 days of gestation). At 7-9 weeks of age, the surviving male rats received orthotopic liver transplantation (OLT) from male LOU/CN donors and the survival period was observed and monitered by mixed lymphocyte reaction assay and cytotoxicity test.

Results: (1) A total of 31 pregnant CHN rats with 172 fetuses received fetal hepatocytes from LOU/CN rats via intrauterine injection. Among them, thirteen pregnant rats showed normal parturition, with 74 neonatal rats growing up normally. (2) The mean survival period after OLT in rats with fetal exposure to fetal hepatocytes was 32.1+/-3.7 days, which was significantly different from the control (11.8+/-2.3 days, P<0.01) in rats without fetal induction of immune tolerance. (3) Mixed lymphocyte proliferation assays yielded remarkable discrepancies between the groups of rats with- or without fetal exposure to fetal hepatocytes, with values of 8,411+/-1,361 and 22,473+/-1,856 (CPM+/-SD, P<0.01) respectively. (4) Cytotoxicity assays showed values of 21.2+/-6.5 % and 64.5+/-7.2 % (P<0.01) in adult rats with or without fetal induction of immune tolerance.

Conclusion: Intrauterine injection of fetal hepatocytes into rat fetuses can prolong the survival period of liver transplant adult male rats recipients, inducting immune tolerance in OLT.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Karim M, Steger U, Bushell AR, Wood KJ. The role of the graft in establishing tolerance. Front Biosci. 2002;7:e129–e154. - PubMed
    1. Riordan SM, Williams R. Transplantation of primary and reversibly immortalized human liver cells and other gene therapies in acute liver failure and decompensated chronic liver disease. World J Gastroenterol. 2000;6:636–642. - PMC - PubMed
    1. Tang ZY. Hepatocellular carcinoma--cause, treatment and metastasis. World J Gastroenterol. 2001;7:445–454. - PMC - PubMed
    1. Zhu XF, Chen GH, He XS, Lu MQ, Wang GD, Cai CJ, Yang Y, Huang JF. Liver transplantation and artificial liver support in fulminant hepatic failure. World J Gastroenterol. 2001;7:566–568. - PMC - PubMed
    1. Platell CF, Coster J, McCauley RD, Hall JC. The management of patients with the short bowel syndrome. World J Gastroenterol. 2002;8:13–20. - PMC - PubMed

Publication types