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. 2003 Jan;41(1):159-63.
doi: 10.1128/JCM.41.1.159-163.2003.

Frequent recovery of a single clonal type of multidrug-resistant Staphylococcus aureus from patients in two hospitals in Taiwan and China

M Aires de Sousa  1 , M I Crisóstomo, I Santos Sanches, J S Wu, J Fuzhong, A Tomasz, H de Lencastre
Affiliations

Frequent recovery of a single clonal type of multidrug-resistant Staphylococcus aureus from patients in two hospitals in Taiwan and China

M Aires de Sousa et al. J Clin Microbiol. 2003 Jan.

Abstract

One hundred thirty-two methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered from patients with S. aureus infections between January 1998 and February 1999 in two hospitals, one located in Taipei, Taiwan, and another in Nanjing, People's Republic of China, were examined for antibiotic susceptibility and for clonal type by a combination of three methods: hybridization of ClaI restriction digests with mecA- and Tn554-specific DNA probes and pulsed-field gel electrophoresis of chromosomal SmaI digests. Selected isolates representing each clonal type were also analyzed by spaA typing, multilocus sequence typing, and a multiplex PCR method capable of identifying the structural type of the staphylococcal cassette chromosome mec (SCCmec) carried by the bacteria. The overwhelming majority of isolates (126 of 132 or 95%) belonged to minor variants of a single clonal type resembling the Brazilian and Hungarian epidemic MRSA clones, which showed a common spaA type and which were either sequence type 239 (ST239) or ST241 (a single-locus variant of ST239) in association with SCCmec type III or IIIA.

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Figures

FIG. 1.
FIG. 1.
(A) PFGE of SmaI macrorestriction fragments of MRSA clinical isolates belonging to the China/Taiwan, Hungarian (7, 18), and Brazilian clones (2, 3, 27). Lanes 1 and 21, lambda molecular weight marker; lanes 2, 11, and 20, reference strain NCTC 8325; lanes 3 to 6, HU25, HSJ216, BRA5, and BRA101, respectively; lanes 7 to 10, HUSA304, HU101, HUSA88, and HUSA176, respectively; lanes 12 to 15, TAW9 (A2), TAW10 (A1), TAW3 (A1), and TAW5 (A2), respectively; lanes 16 to 19, CHI55 (A11), CHI59 (A11), CHI62 (A11), and CHI61 (A14), respectively. (B) Computer-generated dendrogram based on Jaccard matching of pattern similarity of the PFGE of Fig. 1A. The scale at the bottom of the dendrogram represents similarity.
FIG. 1.
FIG. 1.
(A) PFGE of SmaI macrorestriction fragments of MRSA clinical isolates belonging to the China/Taiwan, Hungarian (7, 18), and Brazilian clones (2, 3, 27). Lanes 1 and 21, lambda molecular weight marker; lanes 2, 11, and 20, reference strain NCTC 8325; lanes 3 to 6, HU25, HSJ216, BRA5, and BRA101, respectively; lanes 7 to 10, HUSA304, HU101, HUSA88, and HUSA176, respectively; lanes 12 to 15, TAW9 (A2), TAW10 (A1), TAW3 (A1), and TAW5 (A2), respectively; lanes 16 to 19, CHI55 (A11), CHI59 (A11), CHI62 (A11), and CHI61 (A14), respectively. (B) Computer-generated dendrogram based on Jaccard matching of pattern similarity of the PFGE of Fig. 1A. The scale at the bottom of the dendrogram represents similarity.
See this image and copyright information in PMC

References

    1. Aires de Sousa, M., H. de Lencastre, I. Santos Sanches, K. Kikuchi, K. Totsuka, and A. Tomasz. 2000. Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York City and in a hospital in Tokyo, Japan. Microb. Drug Resist. 6:253-258. - PubMed
    1. Aires de Sousa, M., M. Miragaia, I. Santos Sanches, S. Ávila, I. Adamson, S. T. Casagrande, M. C. C. Brandileone, R. Palacio, L. Dell'Acqua, M. Hortal, T. Camou, A. Rossi, M. E. Velazquez-Meza, G. Echaniz-Aviles, F. Solorzano-Santos, I. Heitmann, and H. de Lencastre. 2001. Three-year assessment of methicillin-resistant Staphylococcus aureus clones in Latin America, 1996 to 1998. J. Clin. Microbiol. 39:2197-2205. - PMC - PubMed
    1. Aires de Sousa, M., I. Santos Sanches, M. L. Ferro, M. J. Vaz, Z. Saraiva, T. Tendeiro, J. Serra, and H. de Lencastre. 1998. Intercontinental spread of a multidrug-resistant methicillin-resistant Staphylococcus aureus clone. J. Clin. Microbiol. 36:2590-2596. - PMC - PubMed
    1. Chen, M. L., S. C. Chang, H. J. Pan, P. R. Hsueh, L. S. Yang, S. W. Ho, and K. T. Luh. 1999. Longitudinal analysis of methicillin-resistant Staphylococcus aureus isolates at a teaching hospital in Taiwan. J. Formos. Med. Assoc. 98:426-432. - PubMed
    1. de Lencastre, H., I. Couto, I. Santos, J. Melo-Cristino, A. Torres-Pereira, and A. Tomasz. 1994. Methicillin-resistant Staphylococcus aureus disease in a Portuguese hospital: characterization of clonal types by a combination of DNA typing methods. Eur. J. Clin. Microbiol. Infect. Dis. 13:64-73. - PubMed

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