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. 2003 Jan;10(1):19-21.
doi: 10.1128/cdli.10.1.19-21.2003.

Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies

Norman A Pinsky  1 , Jeanne M Huddleston, Robert M Jacobson, Peter C Wollan, Gregory A Poland
Affiliations

Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies

Norman A Pinsky et al. Clin Diagn Lab Immunol. 2003 Jan.

Abstract

We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4 degrees C until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.

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Figures

FIG. 1.
FIG. 1.
Antibody levels for MMR. Antibody levels for measles (A), mumps (B), and rubella virus (C) are displayed along the x axis, and the number of freeze-thaw cycles is displayed along the y axis. The horizontal dashed line in each panel indicates the cutoff point, above which results represent seropositivity as established by the kit manufacturer.
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