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Comment
. 2003 Jan;84(1):725-6.
doi: 10.1016/S0006-3495(03)74891-7.

A closer look at the canonical 'Raft Mixture' in model membrane studies

Sarah L Veatch, Sarah L Keller
Comment

A closer look at the canonical 'Raft Mixture' in model membrane studies

Sarah L Veatch et al. Biophys J. 2003 Jan.
No abstract available

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Figures

FIGURE 1
FIGURE 1
(a) Simplified sketch of anticipated miscibility phase diagram for mixtures of 1:1 DOPC/BSM with varying cholesterol composition. The location of the popular mixture of 1:1:1 DOPC/BSM/cholesterol at room temperature (RT) is denoted by a gray circle. (b, c) GUVs of 1:1:1 DOPC/BSM/cholesterol at 25°C (b) and 20°C (c). The same field of view is shown in both frames. In both cases, laterally separated liquid domains are observed in some but not all vesicles. Vesicles with domains are shown with arrows. At 20°C, more vesicles exhibit coexisting domains than at 25°C. (d) All vesicles made with 1:1 DOPC/BSM and 20% cholesterol exhibit phase separation at 25°C. All experiments use the fluorescent dye Texas Red DPPE which preferentially partitions into the cholesterol-poor phase.
See this image and copyright information in PMC

Comment on

References

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