Dorsal raphe nucleus modulates neuronal activity in rat intergeniculate leaflet

Abstract

Serotonergic input from midbrain raphe nuclei is believed to have a significant effect on mammalian circadian timing system. The suprachiasmatic nucleus (SCN) receives its serotonergic input from the median raphe nucleus, while the intergeniculate leaflet (IGL) receives serotonergic innervation from the dorsal raphe nucleus (DRN). The present paper was aimed at determining whether projection from the DRN affected rhythmic neuronal oscillations in the IGL of rats. We investigated the impact of electrolytic lesions and electric stimulation of the DRN on spontaneous isoperiodic (i.e. burst firing with a constant interburst interval) neuronal activity recorded in the IGL. In all our experiments a complete lesion of the DRN always caused a significant increase (ca. 100%) of spontaneous activity of IGL neurons, their oscillatory character having been maintained, though. On the other hand, electric stimulation of the DRN produced a transient decrease in firing rate oscillations of the IGL neurons. The obtained results indicate that the neuronal projection from the DRN has a substantial modulating effect on IGL activity-an important element of the mechanism of the circadian time-keeping system that mediates the transfer of non-photic information to the SCN by modulating its activity. The observed increase of isoperiodic activity in the IGL after DRN lesion and a transient decrease in this activity after electric stimulation indicate an inhibitory character of this effect. The present findings corroborate the hypothesis that the DRN is a one of the major and extremely important source of the modulatory inputs to the mammalian circadian time-keeping system.