[Studies on polysaccharide of Cynomorium songaricum Rupr]

Abstract

Objective: To study the acidic heteropolysac charides of Cynomorium songaricum.

Method: The polysaccharides were purified by Sephadex G-100 and G-150 gel column chromatography. Purity and molecular weight of the polysaccharides were determined by high performance gel permeation chromatography; neutral sugars composition were identified by PC, TLC and GC. Uronic acids were determined by carbazole method.

Result: The molecular weight of SYP-A and SYP-B were estimated to be 3.1 x 10(5) and 2.8 x 10(5) respectively, and neutral sugars were composed of galactose, glucose, arabinose, rhamnose, mannose and ribose. The molar ratio for SYP-A and SYP-B were 5.1:4.1:1.6:1.0:0.5:0.3 and 5.2:4.2:1.5:1.0:0.5:0.2 respectively, The contents of uronic acids were 10.7% and 10.5% respectively.

Conclusion: SYP-A and SYP-B are homogeneous acidic heteropolysaccharides.