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. 2003 Feb;9(2):300-3.
doi: 10.3748/wjg.v9.i2.300.

Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus

Ke Li  1 , Lin Wang, Jun Cheng, Yin-Ying Lu, Ling-Xin Zhang, Jin-Song Mu, Yuan Hong, Yan Liu, Hui-Juan Duan, Gang Wang, Li Li, Ju-Mei Chen
Affiliations

Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus

Ke Li et al. World J Gastroenterol. 2003 Feb.

Abstract

Aim: To investigate the interaction between hepatitis C virus core protein and translin protein and its role in the pathogenensis of hepatocellular carcinoma and lymphoma.

Methods: With the components of the yeast two hybrid system 3, "bait" plasmids of HCV core the gene was constructed. After proving that hepatitis C virus core protein could be firmly expressed in AH109 yeast strains, yeast two- hybrid screening was performed by mating AH109 with Y187 that transformed with liver cDNA library plasmids-pACT2 and then plated on quadruple dropout (QDO) medium and then assayed for alpha-gal activity. Sequencing analysis of the genes of library plasmids in yeast colonies that could grow on QDO with alpha-gal activity was performed. The interaction between HCV core protein and the protein we obtained from positive colony was further confirmed by repeating yeast two - hybrid analysis and coimmunoprecipitation in vitro.

Results: A gene from a positive colony was the gene of translin, a recombination hotspot binding protein. The interaction between HCV core protein and translin protein could be proved not only in yeast, but also in vitro.

Conclusion: The core protein of HCV can interact with translin protein. This can partly explain the molecular mechanism for hepatocellular carcinoma and lymphoma caused by HCV.

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Figures

Figure 1
Figure 1
“bait” plasmid pGBKT7-core.
Figure 2
Figure 2
To confirm the interaction.
Figure 3
Figure 3
Western blotting showed the expression of HCV core protein in yeast (arrow indicated). lane 1 is HCV core protein and lane 2 is negative control.
Figure 4
Figure 4
A 687bp fragment-translin, amplified by RT-PCR. (A)pGADT7-translin cut by EcoRI/BamHI (B).
Figure 5
Figure 5
lane 1 HCV core protein and translin protein, lane 2 HCV core protein.
See this image and copyright information in PMC

References

    1. Harada S, Watanabe Y, Takeuchi K, Suzuki T, Katayama T, Takebe Y, Saito I, Miyamura T. Expression of processed core protein of hepatitis C virus in mammalian cells. J Virol. 1991;65:3015–3021. - PMC - PubMed
    1. Santolini E, Migliaccio G, La Monica N. Biosynthesis and biochemical properties of the hepatitis C virus core protein. J Virol. 1994;68:3631–3641. - PMC - PubMed
    1. Lo SY, Selby M, Tong M, Ou JH. Comparative studies of the core gene products of two different hepatitis C virus isolates: two alternative forms determined by a single amino acid substitution. Virology. 1994;199:124–131. - PubMed
    1. Liu Q, Tackney C, Bhat RA, Prince AM, Zhang P. Regulated processing of hepatitis C virus core protein is linked to subcellular localization. J Virol. 1997;71:657–662. - PMC - PubMed
    1. Yasui K, Wakita T, Tsukiyama-Kohara K, Funahashi SI, Ichikawa M, Kajita T, Moradpour D, Wands JR, Kohara M. The native form and maturation process of hepatitis C virus core protein. J Virol. 1998;72:6048–6055. - PMC - PubMed

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