Review
doi: 10.1016/s1359-6446(02)02474-1.
RNAi: gene-silencing in therapeutic intervention
Affiliations
- PMID: 12546893
- DOI: 10.1016/s1359-6446(02)02474-1
Item in Clipboard
Review
RNAi: gene-silencing in therapeutic intervention
Drug Discov Today.
.
Abstract
Several rapidly developing RNA interference (RNAi) methodologies hold the promise to selectively inhibit gene expression in mammals. RNAi is an innate cellular process activated when a double-stranded RNA (dsRNA) molecule of greater than 19 duplex nucleotides enters the cell, causing the degradation of not only the invading dsRNA molecule, but also single-stranded (ssRNAs) RNAs of identical sequences, including endogenous mRNAs. As such, RNAi technology is currently being evaluated not only as an extremely powerful instrument for functional genomic analyses, but also as a potentially useful method to develop highly specific dsRNA based gene-silencing therapeutics.
Similar articles
-
RNA interference: potential therapeutic targets.Appl Microbiol Biotechnol. 2004 Nov;65(6):649-57. doi: 10.1007/s00253-004-1732-1. Epub 2004 Sep 15. Appl Microbiol Biotechnol. 2004. PMID: 15372214 Review.
-
An update on RNA interference-mediated gene silencing in cancer therapy.Expert Opin Biol Ther. 2014 Nov;14(11):1581-92. doi: 10.1517/14712598.2014.935334. Epub 2014 Jul 10. Expert Opin Biol Ther. 2014. PMID: 25010067 Review.
-
Running interference: prospects and obstacles to using small interfering RNAs as small molecule drugs.Annu Rev Biomed Eng. 2006;8:377-402. doi: 10.1146/annurev.bioeng.8.061505.095848. Annu Rev Biomed Eng. 2006. PMID: 16834561 Review.
-
siRNA as a route to new cancer therapies.Expert Opin Biol Ther. 2005 Feb;5(2):221-4. doi: 10.1517/14712598.5.2.221. Expert Opin Biol Ther. 2005. PMID: 15757383 Review.
-
Small RNA: can RNA interference be exploited for therapy?Lancet. 2003 Oct 25;362(9393):1401-3. doi: 10.1016/S0140-6736(03)14637-5. Lancet. 2003. PMID: 14585643 Review.
Cited by
-
A Comparison of Target Gene Silencing using Synthetically Modified siRNA and shRNA That Express Recombinant Lentiviral Vectors.Acta Naturae. 2009 Jul;1(2):86-90. Acta Naturae. 2009. PMID: 22649608 Free PMC article.
-
Ets1 as a marker of malignant potential in gastric carcinoma.World J Gastroenterol. 2003 Oct;9(10):2154-9. doi: 10.3748/wjg.v9.i10.2154. World J Gastroenterol. 2003. PMID: 14562368 Free PMC article.
-
Downregulation of the adenosine a2b receptor by RNA interference inhibits hepatocellular carcinoma cell growth.ISRN Oncol. 2011;2011:875684. doi: 10.5402/2011/875684. Epub 2011 Apr 28. ISRN Oncol. 2011. PMID: 22091434 Free PMC article.
-
Construction of simple and efficient siRNA validation systems for screening and identification of effective RNAi-targeted sequences from mammalian genes.Methods Mol Biol. 2014;1101:321-38. doi: 10.1007/978-1-62703-721-1_15. Methods Mol Biol. 2014. PMID: 24233788 Free PMC article.
-
RNA Interference Applied to Crustacean Aquaculture.Biomolecules. 2024 Oct 25;14(11):1358. doi: 10.3390/biom14111358. Biomolecules. 2024. PMID: 39595535 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
