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. 1976 Feb;3(2):110-2.
doi: 10.1128/jcm.3.2.110-112.1976.

Cholesterol requirement of mycoplasmas as determined by microtiter test using polyene antibiotics

M W Grabowski, S Rottem, M F Barile

Cholesterol requirement of mycoplasmas as determined by microtiter test using polyene antibiotics

M W Grabowski et al. J Clin Microbiol. 1976 Feb.

Abstract

A microtiter metabolic inhibition test was used to determine the effect of filipin and lucensomycin on the growth of representative species of Mycoplasma and Acholeplasma. The cholesterol-requiring species tested were found to be very susceptible to the two antibiotics, whereas the cholesterol nonrequiring species were not. The utilization of this method for differentiation between the Mycoplasma and Acholeplasma species is suggested and its advantages are discussed.

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