Evaluation of the rapid decarboxylase and dihydrolase test for the differentiation of nonfermentative bacteria
- PMID: 1254712
- PMCID: PMC274249
- DOI: 10.1128/jcm.3.2.137-142.1976
Evaluation of the rapid decarboxylase and dihydrolase test for the differentiation of nonfermentative bacteria
Abstract
A rapid medium for the detection of lysine and ornithine decarboxylase and arginine dihydrolase activity of 439 strains of gram-negative, nonfermenting bacteria was evaluated and compared with Moeller decarboxylase medium. Results were obtained in 4 to 24 h using the rapid medium, whereas Moeller medium often required extended (3 to 7 days) incubation. There was 100% agreement in the lysine tests with both media and almost 100% agreement in the ornithine tests. There was 91% agreement in the arginine tests, with the significance of discrepant results discussed. The sensitivity, specificity, and quick results obtained by the rapid test make it a suitable substitute for Moeller medium for the identication of gram-negative, nonfermenting bacteria.
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