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. 2001 Apr;41(2):248-51.

[Cloning and expression of D-arabitol dehydrogenase gene from Acetobacter suboxydans in Escherichia coli]

[Article in Chinese]
X He  1 , W Huai, W Guo, B Zhang
Affiliations
  • PMID: 12549035

[Cloning and expression of D-arabitol dehydrogenase gene from Acetobacter suboxydans in Escherichia coli]

[Article in Chinese]
X He et al. Wei Sheng Wu Xue Bao. 2001 Apr.

Abstract

The partial genomic library of Acetobacter suboxydans was constructed using Yeast-E. coli shuttle plasmid YEp352 as vector. Two positive transformants, designated as DH5 alpha(pAD91) and DH5 alpha(pAD98), were obtained by screening the growth of transformants on the agar plate in which D-arabitol was used as the sole carbon source. The results of Southern blot and restriction endonuclease analysis showed that the two recombinants are identical. The insert is about 2.3 kb. Arabitol dehydrogenase activity assay indicated that the transformants could produce D-xylulose-forming D-arabitol dehydrogenase. Hence, the gene encoding D-arabitol dehydrogenase exists in the cloned DNA fragment.

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