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. 1998 Apr;38(2):142-5.

[Sequencing of a beta-amylase gene from Bacillus firmus]

[Article in Chinese]
Affiliations
  • PMID: 12549376

[Sequencing of a beta-amylase gene from Bacillus firmus]

[Article in Chinese]
W Chen et al. Wei Sheng Wu Xue Bao. 1998 Apr.

Abstract

The gene encoding a beta-amylase from Bacillus firmus 725 was sequenced. The sequenced DNA of 2012 bp contains one open reading frame of 1406 nucleotides without a translation stop codon. The deduced amino acid sequence homology with those known bacterial and some plant beta-amylase was 98% for Bacillus polymyxa 72, 98% for Bacillus polymyxa ATCC8523, 82% for Bacillus circulans, 54% for Clostridium thermosulfurogenes, 49% for Bacillus cereus BQ10-S1, 50% for Bacillus cereus var. mycoides, 36% for barley, and 36% for soybean Eleven well-conserved regions were found among the amino acid sequences of the nine beta-amylases.

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