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. 1998 Oct;38(5):393-5.

[Establishment of hybridoma secreting anti-mycobacteria monoclonal antibody by using electrofusion technique]

[Article in Chinese]
M Guo  1 , Z Pan, H Wang
Affiliations
  • PMID: 12549406

[Establishment of hybridoma secreting anti-mycobacteria monoclonal antibody by using electrofusion technique]

[Article in Chinese]
M Guo et al. Wei Sheng Wu Xue Bao. 1998 Oct.

Abstract

By using the electrofusion technique, the fusion of NS1 myeloma cells with spleen cells of BALB/c mouse immunized with five species of mycobacteria was carried out. With the optimal electrofusion conditions, we obtained a high fusion efficiency of 1.75 hybrid cell clones per 10(5) spleen cells. ELISA was used for screening secreting antimycobacteria McAb hybrid cells. After cloning, 22 stable hybrid cells lines were selected, and 8 of them had been studied.

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