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. 2001 Aug;41(4):402-7.

[Cloning and sequencing of the gene encoding esterase estA from Ralstonia eutropha CH34]

[Article in Chinese]
S Song  1 , A Steinbüchel
Affiliations
  • PMID: 12552903

[Cloning and sequencing of the gene encoding esterase estA from Ralstonia eutropha CH34]

[Article in Chinese]
S Song et al. Wei Sheng Wu Xue Bao. 2001 Aug.

Abstract

An esterase-positive clone was isolated by screening a genomic library of Ralstonia eutropha CH34 constructed in Escherichia coli S17-1 with top agar containing alpha-naphtyl acetate and Fast Blue RR. A gne encoding esterase activity, estA was subcloned from this clone. Nucleotide sequencing of estA showed that it was a 825 bp open reading frame, encoding an esterase EstA, composed of 275 amino acids with a predicted molecular mass of 30,785 D. Homology analysis revealed that EstA exhibited significant amino acid similarity with the enzymes involved in the meta-cleavage pathway in the metaboleism of aromatic acid compounds.

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