Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 May 15;372(Pt 1):235-40.
doi: 10.1042/BJ20021202.

U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5' splice sites

Patrik Förch  1 , Livia Merendino, Concepción Martínez, Juan Valcárcel
Affiliations

U2 small nuclear ribonucleoprotein particle (snRNP) auxiliary factor of 65 kDa, U2AF65, can promote U1 snRNP recruitment to 5' splice sites

Patrik Förch et al. Biochem J. .

Abstract

The splicing factor U2AF(65), U2 small nuclear ribonucleoprotein particle (snRNP) auxillary factor of 65 kDa, binds to pyrimidine-rich sequences at 3' splice sites to recruit U2 snRNP to pre-mRNAs. We report that U2AF(65) can also promote the recruitment of U1 snRNP to weak 5' splice sites that are followed by uridine-rich sequences. The arginine- and serine-rich domain of U2AF(65) is critical for U1 recruitment, and we discuss the role of its RNA-RNA annealing activity in this novel function of U2AF(65).

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1999 Dec 16;402(6763):832-5 - PubMed
    1. Genes Dev. 1999 Mar 1;13(5):569-80 - PubMed
    1. Nature. 1999 Dec 16;402(6763):838-41 - PubMed
    1. Mol Cell Biol. 2000 Sep;20(17):6287-99 - PubMed
    1. J Biol Chem. 2000 Dec 1;275(48):38059-66 - PubMed

Publication types