[Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator]
- PMID: 12561197
[Regulation of Gal beta 1,3GalNAc alpha 2,3-sialyltransferase (ST3GalI) by hepatitis B virus MHBst/HBx transactivator]
Abstract
Hepatitis B virus MHBst and HBx fragments were amplified to construct eukaryotic expression vector pCDNA3.1-MHBst and pCDNA3.1-HBx. ST3GalI promoter region was obtained by the method of PCR and GFP report plasmid pEGFP-N1-Psial was constructed. pCDNA3.1-MHBst or pCDNA3.1-HBx with pEGFP-N1-Psial were transiently co-transfected into QGY-7701 cells using calcium phosphate-DNA co-precipitation, respectively. The expressions of Psial-directed GFP were analyzed by FAC-Scalibur. It was found that MHBst/HBx could upregulate ST3GalI promoter activity by 35.2% and 43.8%, respectively. We report the regulation of ST3GalI by MHBst and HBx transactivators. It would be helpful to further investigate the relation between hepatitis B virus infection and sialyltransferase expression.
Similar articles
-
[Inhibition of HBV DNA replication and expression in 2.2.15 hepatoma cells infected with AFP-mediated HBX antisense RNA].Zhonghua Gan Zang Bing Za Zhi. 2003 May;11(5):291-4. Zhonghua Gan Zang Bing Za Zhi. 2003. PMID: 12773245 Chinese.
-
[Biological impact of the COOH-terminal 40 amino acid deletions of hepatitis B virus X protein in hepatocellular carcinoma cells].Zhonghua Yi Xue Za Zhi. 2005 Mar 30;85(12):825-30. Zhonghua Yi Xue Za Zhi. 2005. PMID: 15949399 Chinese.
-
[Effects of hepatitis B virus X gene on p21(WAF1) expression through p53-dependent and p53-independent pathways].Ai Zheng. 2004 Jul;23(7):749-55. Ai Zheng. 2004. PMID: 15248906 Chinese.
-
[Construction of pEGFP-HBx gene recombinant plasmid and establishment of stable transfected HBx gene liver oval cell strain].Zhonghua Wai Ke Za Zhi. 2008 Dec 15;46(24):1919-22. Zhonghua Wai Ke Za Zhi. 2008. PMID: 19134388 Chinese.
-
[Sialyltransferase superfamily: structure and function].Tanpakushitsu Kakusan Koso. 1998 Dec;43(16 Suppl):2338-48. Tanpakushitsu Kakusan Koso. 1998. PMID: 9883657 Review. Japanese. No abstract available.