[Cloning and sequence analysis of histidine-rich protein-II gene fragment of Plasmodium falciparum yunnan strain]

Abstract

Aim: To determine the nucleotide sequence of the partial gene of the histidine-rich protein II (HRP II) the Plasmodium falciparum PFD-3/YN and find out the differences of the HRP II gene sequence between this isolate and other isolates.

Methods: The histidine-rich protein-II gene fragment was amplified by polymerase chain reaction and cloned into M13 bacteriophage. M13-HRP II single strand DNAs of three positive clones were extracted, respectively. The nucleotide sequence of the HRP II gene fragment was determined by the dideoxy chain termination method. Using PCGENE software to compare and analyze the HRP II gene sequence among the different isolates.

Results: Different degrees of diversity of the HRP II gene sequences were found among Plasmodium falciparum PFD-3/YN(from China) and two other isolates (7G8 from Brazil and D10 from Gambia). The HRP II in the three isolates exhibited 70.3% homology in amino acid sequences and 68.6% homology in the nucleic acid sequences.

Conclusion: There were differences in HRP II gene sequence among the Plasmodium falciparum PFD-3/YN and two other isolates (7G8 and D10).