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. 1976 Feb;3(2):381-91.
doi: 10.1093/nar/3.2.381.

Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide

P T Lomedico, G F Saunders
Free PMC article

Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide

P T Lomedico et al. Nucleic Acids Res. 1976 Feb.
Free PMC article

Abstract

Total nucleic acid extraction and selection of poly A-containing molecules yield preparative quantities of undegraded mRNA from adult and fetal pancreas. Using a stringent immunoassay, this mRNA is found to direct the synthesis of an immunoreactive insulin polypeptide in the wheat germ translation system. On sodium dodecyl sulfate polyacrylamide gels, this polypeptide (12,000-13,000 daltons) is larger than proinsulin (9,000 daltons).

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