Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001;19(2):103-6.

[Studies on detecting Brugia malayi larva in mosquitoes by polymerase chain reaction]

[Article in Chinese]
Y Wang  1 , X D Dai, X G Tian, Y Cui, J Li, X D Yuan, D J Sun
Affiliations
  • PMID: 12571998

[Studies on detecting Brugia malayi larva in mosquitoes by polymerase chain reaction]

[Article in Chinese]
Y Wang et al. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2001.

Abstract

Objective: To establish a specific, sensitive and simple assay for the detection of Brugia malayi larva in Anopheles sinensis.

Methods: Using a new DNA purification technique (Microcon 100) and two pairs of oligonucleotide primers (p1, p2 and p3, p4) suitable for detecting B. malayi in seven areas in our country, the mosquito vectors infected by B. malayi were detected by polymerase chain reaction(PCR).

Results: This PCR method could amplify separately a 322-basepair(bp) and a 155 bp DNA fragment and detect as few as 1/64 of one L1 in 1 mosquito, the detectable limit was nearly 4 pg DNA of filarial larvae, and it could also detect 1 infected mosquito with one L3 of B. malayi in pools of up to 200 mosquitoes. In contrast, no such specific 322 bp or 155 bp DNA band was detected in Dilofilaria immitis and normal mosquito.

Conclusion: This PCR technique established for supervision of mosquito vector in B. malayi endemic areas is specific, sensitive, and simple.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources