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. 1976 Mar;151(3):587-90.
doi: 10.3181/00379727-151-39265.

In vitro colony assay for a new class of megakaryocyte precursor: colony-forming unit megakaryocyte (CFU-M)

A Nakeff, S Daniels-McQueen

In vitro colony assay for a new class of megakaryocyte precursor: colony-forming unit megakaryocyte (CFU-M)

A Nakeff et al. Proc Soc Exp Biol Med. 1976 Mar.

Abstract

A plasma culture system has been used successfully to grow and quantitate megakaryocyte colonies from mouse bone marrow following their staining for acetylcholinesterase activity in situ. Colonies averaging about six acetylcholinesterase-positive cells appear with a peak incidence after 4 days in culture with a plating efficiency of one colony formed for every 10(4) nucleated cells plated.

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