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. 2003 Feb;41(2):813-5.
doi: 10.1128/JCM.41.2.813-815.2003.

Detection of Helicobacter pylori in paraffin-embedded and in shock-frozen gastric biopsy samples by fluorescent in situ hybridization

Holger Rüssmann  1 , Anne Feydt-Schmidt, Kristin Adler, Daniela Aust, Almuth Fischer, Sibylle Koletzko
Affiliations

Detection of Helicobacter pylori in paraffin-embedded and in shock-frozen gastric biopsy samples by fluorescent in situ hybridization

Holger Rüssmann et al. J Clin Microbiol. 2003 Feb.

Abstract

We report on the successful application of fluorescent in situ hybridization for detection of Helicobacter pylori and determination of its clarithromycin susceptibility in formalin-fixed and paraffin-embedded gastric biopsy specimens that had been prepared for pathological examination. This method is useful when results from conventional culturing with antibiotic susceptibility testing are not available.

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Figures

FIG. 1.
FIG. 1.
Application of FISH for H. pylori detection in formalin-fixed and deparaffinized gastric biopsy specimens and in shock-frozen gastric biopsy specimens from 100 dyspeptic patients. UBT, 13C-urease breath test; RUT, rapid urease test; Cla-S, clarithromycin sensitive; Cla-R, clarithromycin resistant.
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References

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