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. 2003 Feb;41(2):826-30.
doi: 10.1128/JCM.41.2.826-830.2003.

Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

Béatrice Ninet  1 , Isabelle Jan, Olympia Bontems, Barbara Léchenne, Olivier Jousson, Renato Panizzon, Daniel Lew, Michel Monod
Affiliations

Identification of dermatophyte species by 28S ribosomal DNA sequencing with a commercial kit

Béatrice Ninet et al. J Clin Microbiol. 2003 Feb.

Abstract

We have shown that dermatophyte species can be easily identified on the basis of a DNA sequence encoding a part of the large-subunit (LSU) rRNA (28S rRNA) by using the MicroSeq D2 LSU rRNA Fungal Sequencing Kit. Two taxa causing distinct dermatophytoses were clearly distinguished among isolates of the Trichophyton mentagrophytes species complex.

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Figures

FIG. 1.
FIG. 1.
Multiple alignment of partial 28S rDNA sequences of Trichophyton (T), Microsporum (M), and Epidermophyton (E) species. A dash indicates an alignment gap; a dot indicates the same base as on the upper line.
FIG. 2.
FIG. 2.
Alignment of multiple complete ITS rDNA sequences (ITS1 plus 5.8S plus ITS2) of T. mentagrophytes types I, II, III, and IV (accession no. AF506033, AF506036, AF506034, and A4185126, respectively) and T. tonsurans (AF170478). The sequence with accession no. AF506033 was found in four isolates of T. mentagrophytes type I and one isolate of type II. The sequence with accession no. AF506036 was found in three isolates of type I and two isolates of type II. The sequence with accession no. AF506034 was found in the five isolates of T. mentagrophytes type III tested. ITS1, bp 23 to 284; 5.8S, bp 285 to 440; ITS2, bp 441 to 623.
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