[Transperitoneal transport of solute and IL-8 in dialysis-related peritonitis]

Abstract

Objective: To study the changes in solute transperitoneal transport and the IL-8 level at different time phases in dialysis-related peritonitis so as to understand the mechanism of solute transperitoneal transport.

Methods: Forty-two New Zealand white rabbits were randomly divided into three experimental groups (5 min, 24 h, and 48 h groups) and a control group. The rabbit model of peritoneal dialysis-related peritonitis was created. The plasma and effluent concentration of creatinine, glucose, total proteins, and albumin were determined respectively; the D/P values of creatinine, total proteins, and albumin, and the Dn/D0 values of glucose were calculated respectively.

Results: There was a significant difference in the D/P values of albumin at 120, 240, and 360 min between the 48 h group and the control group (P < 0.05); the D/P values of total proteins significantly increased at different time points in the 48 h group compared with the control group (P < 0.05); there was a significant difference in the creatinine D/P values and glucose Dn/D0 values between the 48 h group and the control group (P < 0.05). Compared with the control group, IL-8 and WBC obviously increased in the 48 h group (P < 0.05); the effluent IL-8 levels at different time points were positively correlated to the D/P values of the total proteins (P < 0.05). The effluent IL-8 levels at different time points after 60 min were positively correlated to the D/P values of albumin (P < 0.05).

Conclusion: The mechanism of transperitoneal transport of the different molecule solutes varies. Both IL-8 and WBC can influence solute transperitoneal transport in peritoneal dialysis-related peritonitis.