Nitrate removal by simultaneous sulfur utilizing autotrophic and heterotrophic denitrification under different organics and alkalinity conditions: batch experiments

Abstract

The effect of various organic compounds were tested using lab-scale batch reactors. At sufficient alkalinity, the initial nitrate nitrogen concentration of 100 mg/L was completely reduced in all batch reactors. Sulfate production decreased by the addition of organics. The concentration range of organics used in this experiment did not inhibit autotrophic denitrification except for propionate. Propionate inhibited autotrophic denitrification a little, indicated by a lower sulfate production rate. Biomass in suspension increased with higher initial organic concentrations, showing higher DOC consumption. As the concentration of organics increased, alkalinity increased accordingly. Under the conditions of low alkalinity, in the case of a control reactor without organics, only about 30% of the initial nitrate was reduced. With half the theoretically required dosage of methanol, the denitrification rates increased slightly. When ethanol, acetate, and propionate were used, denitrification went to completion. When excess organics was added, however, sulfate production was significantly decreased. Interestingly, even when small amounts of organics were added, autotrophic denitrification was promoted as indicated by the sulfate production.