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Review
. 2003 Feb 17;1610(1):3-10.
doi: 10.1016/s0005-2736(02)00707-1.

Assembly and overexpression of membrane proteins in Escherichia coli

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Free article
Review

Assembly and overexpression of membrane proteins in Escherichia coli

David Drew et al. Biochim Biophys Acta. .
Free article

Abstract

The bacterium Escherichia coli is one of the most popular model systems to study the assembly of membrane proteins of the so-called helix-bundle class. Here, based on this system, we review and discuss what is currently known about the assembly of these membrane proteins. In addition, we will briefly review and discuss how E. coli has been used as a vehicle for the overexpression of membrane proteins.

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