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. 2003 Jan 28;218(2):223-30.
doi: 10.1016/S0378-1097(02)01148-5.

Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602

Andreas Rascher  1 , Zhihao Hu, Nina Viswanathan, Andreas Schirmer, Ralph Reid, William C Nierman, Matthew Lewis, C Richard Hutchinson
Affiliations

Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602

Andreas Rascher et al. FEMS Microbiol Lett. .

Abstract

We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602.

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