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. 2003 Jan;13(1):29-37.
doi: 10.2188/jea.13.29.

The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents

Koji Suzuki  1 , Yoshinori Ito, Junichi Ochiai, Kunio Aoki, Kenji Wakai, Akiko Tamakoshi, Masahiko Ando, Yoshiyuki Watanabe, Kotaro Ozasa, Nao Seki, Yoshikazu Nishino, Takaaki Kondo, Yoshiyuki Ohno, Akiko Tamakoshi, Mitsuru Mori, Yutaka Motohashi, Ichiro Tsuji, Yosikazu Nakamura, Hiroyasu Iso, Haruo Mikami, Shuji Hashimoto, Yutaka Inaba, Yoshiharu Hoshiyama, Hiroshi Suzuki, Hiroyuki Shimizu, Hideaki Toyoshima, Shinkan Tokudome, Yoshinori Ito, Shogo Kikuchi, Akio Koizumi, Takashi Kawamura, Yoshiyuki Watanabe, Tsuneharu Miki, Chigusa Date, Kiyomi Sakata, Takayuki Nose, Norihiko Hayakawa, Takesumi Yoshimura, Katsuhiro Fukuda, Naoyuki Okamoto, Hideo Shio, Yoshiyuki Ohno, Tomoyuki Kitagawa, Toshio Kuroki, Kazuo Tajima; Japan Collaborative Cohort Study Group
Affiliations

The relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies, Mn-SOD and carotenoids in rural Japanese residents

Koji Suzuki et al. J Epidemiol. 2003 Jan.

Abstract

This study investigated the relationship between smoking habits and serum levels of 8-OHdG, oxidized LDL antibodies (oLAB), Mn-SOD, and carotenoids. Subjects were 79 males (mean age +/- standard deviation; 62.1 +/- 10.0 years) and 79 females (60.3 +/- 10.3 y) who attended a health examination screening in the town of Hokkaido, Japan. Serum 8-OHdG, Mn-SOD, and oLAB levels were measured by ELISA and serum carotenoids levels were measured by HPLC. Smoking habits were assessed by public health nurses using a questionnaire. Serum 8-OHdG levels were significantly higher in males than in females. On the other hand, serum levels of beta-carotene, alpha-carotene, beta-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males than in females. Serum beta-carotene, beta-cryptoxanthin, and zeaxanthin and lutein were significantly lower in males who were current smokers, compared to non-smokers. Serum 8-OHdG levels were also significantly higher in current smokers. Furthermore, in males, serum oLAB and beta-carotene levels were significantly and negatively correlated with the number of cigarettes smoked per day. Serum Mn-SOD levels were unrelated to smoking habits in males. In conclusion, this preliminary study suggests that cigarette smoking increases serum 8-OHdG levels and reduces serum levels of oLAB and carotenoids, such as beta-carotene, beta-cryptoxanthin, and zeaxanthin and lutein in healthy male subjects. Serum levels of 8-OHdG, oLAB, and carotenoids may be useful biomarkers of oxidative conditions affected by smoking.

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References

    1. Kiyosawa H, Suko M, Okudaira H, Murata K, Miyamoto T, Chung MH, et al. . Cigarette smoking induces formation of 8-hydroxydeoxyguanosine, one of the oxidative DNA damages in human peripheral leukocytes. Free Radic Res Commun 1990;11:23-7. - PubMed
    1. Saintot M, Astre C, Pujol H, Gerber M. Tumor progression and oxidant-antioxidant status. Carcinogenesis 1996;17:1267-71. - PubMed
    1. Shibutani S, Takeshita M, Grollman AP. Insertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG. Nature 1991;349:431-4. - PubMed
    1. Bendich A, Olson JA. Biological actions of carotenoids. FASEB J 1989;3:1927-32. - PubMed
    1. Aoki K, Ito Y, Sasaki R, Ohtani M, Hamajima N, Asano A. Smoking, alcohol drinking and serum carotenoids levels. Jpn J Cancer Res 1987;78:1049-56. - PubMed

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