A novel nanoflow interface for atmospheric pressure ionization mass spectrometry

Abstract

A novel spray-ionization technique for nanoflow liquid chromatography/mass spectrometry (nLC/MS) has been developed by modifying the sonic spray ionization (SSI) technique. A solution from a tapered fused-silica capillary is sprayed by a gas flow coaxial to the capillary, and ions produced are analyzed with an ion-trap mass spectrometer. The ion intensity is shown to have a steep threshold at a low gas velocity and to be much less dependent on the gas velocity than that of conventional SSI, in which the ion intensity is strongly dependent on the gas velocity and reaches its maximum at sonic velocity. Thus, we conclude that the concentration of charge in the solution at the tapered capillary tip with an inner diameter of 15 microm is almost at saturation so that charged droplets are produced from the solution by electrical force, rather than by sheer stress due to the gas flow. The ions are readily produced from these charged droplets. Preliminary results are compared with results obtained with a miniaturized electrospray unit.