doi: 10.1007/BF00446839.
Preparation and partial characterization of a Lactobacillus lactis bacteriophage
- PMID: 1259512
- DOI: 10.1007/BF00446839
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Preparation and partial characterization of a Lactobacillus lactis bacteriophage
Arch Microbiol.
.
Abstract
High-titer lysates of a bacteriophage active against Lactobacillus lactis were prepared from liquid cultures as well as from areas of confluent lysis in soft-agar overlayers. Phage concentration and purification were accomplished by means of polyethylene glycol precipitation, differential centrifugation. The buoyant density of this phage in cesium chloride was 1.4795 g/ml. Characterization of phage growth cycle by one-step growth experiments under optimal conditions showed that the latent period was about 120 min, that the rise period lasted approx. 130 min, and that the average burst-size was about 80.
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