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. 1976 Mar 19;107(2).
doi: 10.1007/BF00446839.

Preparation and partial characterization of a Lactobacillus lactis bacteriophage

S S Sarimo, M Hartiala, L Aaltonen

Preparation and partial characterization of a Lactobacillus lactis bacteriophage

S S Sarimo et al. Arch Microbiol. .

Abstract

High-titer lysates of a bacteriophage active against Lactobacillus lactis were prepared from liquid cultures as well as from areas of confluent lysis in soft-agar overlayers. Phage concentration and purification were accomplished by means of polyethylene glycol precipitation, differential centrifugation. The buoyant density of this phage in cesium chloride was 1.4795 g/ml. Characterization of phage growth cycle by one-step growth experiments under optimal conditions showed that the latent period was about 120 min, that the rise period lasted approx. 130 min, and that the average burst-size was about 80.

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