Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Mar 5;125(9):2416-25.
doi: 10.1021/ja026917a.

A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz  1 , Katsuhiko Kamada, Stephen K Burley, David Fenyö, Brian T Chait, Tom W Muir
Affiliations

A modular cross-linking approach for exploring protein interactions

Michelle Trester-Zedlitz et al. J Am Chem Soc. .

Abstract

A method is described for the elucidation of protein-protein interactions using novel cross-linking reagents and mass spectrometry. The method incorporates (1) a modular solid-phase synthetic strategy for generating the cross-linking reagents, (2) enrichment and digestion of cross-linked proteins using microconcentrators, (3) mass spectrometric analysis of cross-linked peptides, and (4) comprehensive computational analysis of the cross-linking data. This integrated approach has been applied to the study of cross-linking between the components of the heterodimeric protein complex negative cofactor 2.

PubMed Disclaimer

Publication types

MeSH terms