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Comparative Study
. 2003 Feb;9(2):224-8.
doi: 10.3201/eid0902.020031.

Aeromonas isolates from human diarrheic stool and groundwater compared by pulsed-field gel electrophoresis

Mark A Borchardt  1 , Mary E Stemper, Jon H Standridge
Affiliations
Comparative Study

Aeromonas isolates from human diarrheic stool and groundwater compared by pulsed-field gel electrophoresis

Mark A Borchardt et al. Emerg Infect Dis. 2003 Feb.

Abstract

Gastrointestinal infections of Aeromonas species are generally considered waterborne; for this reason, Aeromonas hydrophila has been placed on the United States Environmental Protection Agency Contaminant Candidate List of emerging pathogens in drinking water. In this study, we compared pulsed-field gel electrophoresis patterns of Aeromonas isolates from stool specimens of patients with diarrhea with Aeromonas isolates from patients' drinking water. Among 2,565 diarrheic stool specimens submitted to a Wisconsin clinical reference laboratory, 17 (0.66%) tested positive for Aeromonas. Groundwater isolates of Aeromonas were obtained from private wells throughout Wisconsin and the drinking water of Aeromonas-positive patients. The analysis showed that the stool and drinking water isolates were genetically unrelated, suggesting that in this population Aeromonas gastrointestinal infections were not linked with groundwater exposures.

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Figures

Figure 1
Figure 1
Location of Wisconsin residents who submitted diarrheic stool specimens to Marshfield Laboratories. The symbol indicates the location of Marshfield, WI. Symbol size is proportional to the number of specimens. (For reference, the symbol for Marshfield = 208 specimens.)
Figure 2
Figure 2
Pulsed-field gel electrophoresis patterns and similarity dendrogram of genomic DNA from Aeromonas hydrophila and A. caviae isolates from diarrheic stool (S) and groundwater (W). The number refers to the isolate number. DNA molecular weight scale derived from Staphylococcus aureus NCTC 8325.
See this image and copyright information in PMC

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