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. 2003 Mar;47(3):1120-4.
doi: 10.1128/AAC.47.3.1120-1124.2003.

A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus

T M Diaz-Guerra  1 , E Mellado, M Cuenca-Estrella, J L Rodriguez-Tudela
Affiliations

A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus

T M Diaz-Guerra et al. Antimicrob Agents Chemother. 2003 Mar.

Erratum in

  • Antimicrob Agents Chemother. 2004 Mar;48(3):1071

Abstract

The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.

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References

    1. Chryssanthou, E. 1997. In vitro susceptibility of respiratory isolates of Aspergillus species to itraconazole and amphotericin B. acquired resistance to itraconazole. Scand. J. Infect. Dis. 29:509-512. - PubMed
    1. Cove, D. J. 1966. The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. Biophys. Acta 113:51-56. - PubMed
    1. Cuenca-Estrella, M., J. L. Rodriguez-Tudela, E. Mellado, J. V. Martinez-Suarez, and A. Monzon. 1998. Comparison of the in vitro activity of voriconazole (UK-109,496), itraconazole and amphotericin B against clinical isolates of Aspergillus fumigatus. J. Antimicrob. Chemother. 42:531-533. - PubMed
    1. Dannaoui, E., E. Borel, M. F. Monier, M. A. Piens, S. Picot, and F. Persat. 2001. Acquired itraconazole resistance in Aspergillus fumigatus. J. Antimicrob. Chemother. 47:333-340. - PubMed
    1. Dannaoui, E., E. Borel, F. Persat, M. F. Monier, M. A. Piens, the EGBA Network, and the European Research Group on Biotypes and Genotypes of Aspergillus fumigatus. 1999. In-vivo itraconazole resistance of Aspergillus fumigatus in systemic murine aspergillosis. J. Med. Microbiol. 48:1087-1093. - PubMed

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