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Comparative Study
. 2003 Jan-Feb;48(1):4-9.

[Identification of orthopoxvirus species using oligonucleotide micro-chips]

[Article in Russian]
M V Mikheev, S A Lapa, S N Shchelkunov, A K Chikova, V M Mikhaĭlovich, A Iu Sobolev, I V Babkin, D A Griadunov, M A Bulavkina, A A Gus'kov, E B Sokunova, G V Kochneva, V M Blinov, L S Sandakhchiev, A S Zasedatelev, A D Mirzabekov
  • PMID: 12608052
Comparative Study

[Identification of orthopoxvirus species using oligonucleotide micro-chips]

[Article in Russian]
M V Mikheev et al. Vopr Virusol. 2003 Jan-Feb.

Abstract

A method for describing the Orthopoxviruses that are pathogenic both to man and animals is described in the article. The method is based on hybridization of a fluorescently labelled amplified DNA sample with oligonucleotides, which were immobilized in a microchip. Species-specific regions within the crmB gene encoding a viral analogue of the tumor necrosis factor receptor, i.e. an important gene determining the pathogenicity of the mentioned Orthopoxviruses type, were used as a target for identification. The identification procedure takes around 6 hours and does not demand any costly equipment (a portable fluorescent microscope can be used).

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