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. 2003 Apr;53(1):131-5.
doi: 10.1016/s0167-7012(02)00232-4.

Rapid differentiation between short-chain-length and medium-chain-length polyhydroxyalkanoate-accumulating bacteria with spectrofluorometry

Hsan-Au Wu  1 , Der-Shyan Sheu, Chia-Yin Lee
Affiliations

Rapid differentiation between short-chain-length and medium-chain-length polyhydroxyalkanoate-accumulating bacteria with spectrofluorometry

Hsan-Au Wu et al. J Microbiol Methods. 2003 Apr.

Abstract

An approach for rapid differentiation between short-chain-length (scl) and medium-chain-length (mcl) polyhydroxyalkanoate (PHA) producers was developed. Polyhydroxyalkanoate-accumulated bacterial cells stained with Nile red were suspended in water and subjected to fluorescence spectroscopy at a fixed excitation wavelength of 488 nm. The scl-PHA-accumulated bacteria revealed a maximum emission wavelength at 590 nm, and for mcl-PHA producers were seen at a wavelength of 575 nm. Combining Nile red staining and fluorescence spectroscopy, the accumulated PHA granules could be rapidly differentiated into scl-PHA and mcl-PHA from the intact cells.

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