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. 2002 Nov;2(1-2):61-7.
doi: 10.1016/s0925-4773(02)00380-5.

A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis

Nicola Smart  1 , Alison A Hill, James C Cross, Paul R Riley
Affiliations

A differential screen for putative targets of the bHLH transcription factor Hand1 in cardiac morphogenesis

Nicola Smart et al. Gene Expr Patterns. 2002 Nov.

Retraction in

  • Retraction.
    [No authors listed] [No authors listed] Mech Dev. 2002 Dec;119 Suppl 1:S5-10. doi: 10.1016/s0925-4773(03)00085-6. Mech Dev. 2002. PMID: 14524327 No abstract available.

Abstract

The bHLH transcription factor, Hand1 has been implicated in cardiac looping in the mouse, however its function in the developing heart remains unknown. To investigate the mechanism(s) through which Hand1 might function, we screened for potential downstream target genes using representational difference analysis. Thymosin beta4 was found to be down-regulated whereas cystatin C and alphaCA were up-regulated in Hand1-null embryoid bodies. Whole-mount in situ hybridisation on wild type embryos (E8.0-E10.5) and Hand1 homozygous-mutant embryos (E8.0) confirmed co-expression of the putative targets with Hand1 in the heart and their aberrant expression in a Hand1-null background.

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