Fluorescence: basic concepts, practical aspects, and some anecdotes

Abstract

We hope that we have conveyed information of interest and value to present and future fluorescence practitioners. Those readers with a sustaining interest in this topic may wish to consult more comprehensive sources such as Molecular Fluorescence: Principles and Applications, an excellent text by Valeur, or Principles of Fluorescence Spectroscopy by Lakowicz. Many specialized fluorescence topics are covered in the series Topics in Fluorescence Spectroscopy (Volumes 1-6), and several volumes of Methods in Enzymology (e.g., Volumes 246 and 278) have dealt with issues in fluorescence spectroscopy. Proceedings from the International Conference on Methods and Applications of Fluorescence Spectroscopy, 1997 (MAFS 97) and MAFS 98 (in press) also present fluorescence work on many different topics in biological and chemical fields. The Molecular Probes Handbook and web site (www.probes.com) are also rich sources of useful information. Finally, any reader with a question or seeking advice on some topic related to fluorescence is welcome to e-mail D.M.J. at djameson@hawaii.edu.