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. 2003 Feb 15;75(4):875-82.
doi: 10.1021/ac0206317.

High-throughput protein sequencing

Victoria Pham  1 , Jake Tropea, Suzy Wong, James Quach, William J Henzel
Affiliations

High-throughput protein sequencing

Victoria Pham et al. Anal Chem. .

Abstract

We have designed and implemented an autosampler that provides additional sample capacity on a commercial protein sequencer. The autosampler attaches to a standard ABI Procise sequencer, enabling a single-sample cartridge to hold up to six separate samples. The autosampler is used in combination with faster Edman cycles and a rapid 12-min PTH separation to significantly increase the speed of automated protein sequencing. We also describe Edman chemistry modifications that improve coupling efficiency and prevent internal cleavage that can occur when samples are sequenced in the standard glass cartridges.

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