Increased rectal mucosal expression of interleukin 1beta in recently acquired post-infectious irritable bowel syndrome

Abstract

Background and aims: Chronic bowel disturbances resembling irritable bowel syndrome (IBS) develop in approximately 25% of patients after an episode of infectious diarrhoea. Although we have previously shown that psychosocial factors operating at the time of, or prior to, the acute illness appear to predict the development of post-infectious IBS (PI-IBS), our finding of an increased inflammatory cell number in the rectum persisting for at least three months after the acute infection suggested that there is also an organic component involved in the development of PI-IBS. To evaluate this further, we measured expressions of interleukin 1beta (IL-1beta) and its receptor antagonist (IL-1ra) in these patients to provide additional evidence that the pathogenesis of PI-IBS is underpinned by an inflammatory process.

Methods: Sequential rectal biopsy samples were prospectively obtained during and three months after acute gastroenteritis, from eight patients who developed post-infectious IBS (INF-IBS) and seven patients who returned to normal bowel habits after acute gastroenteritis (infection controls, INF-CON). Eighteen healthy volunteers who had not suffered from gastroenteritis in the preceding two years served as normal controls (NOR-CON). IL-1beta and IL-1ra gene expressions were assayed by reverse transcriptase-polymerase chain reaction, and their levels of expression were quantitated by optical densitometry after electrophoresis on agarose gel.

Results: INF-IBS patients exhibited significantly greater expression of IL-1beta mRNA in rectal biopsies than INF-CON patients both during and three months after acute gastroenteritis. Moreover, IL-1beta mRNA expression had increased in biopsies taken from INF-IBS patients at three months after the acute infection but no consistent change was observed in INF-CON patients. IL-1beta mRNA expression of INF-IBS patients at three months post gastroenteritis was significantly greater than NOR-CON whereas that of INF-CON patients was not significantly different from NOR-CON. Despite these differential changes in IL-1beta mRNA expression, no significant changes were observed in IL-1ra mRNA expression among the three groups.

Conclusions: These findings indicate that those patients who develop IBS post infection exhibit greater IL-1beta mRNA expression, both during and after the infection, compared with individuals who do not develop PI-IBS. We conclude that such patients may be susceptible to inflammatory stimuli, and that inflammation may play a role in the pathogenesis of PI-IBS.

Figure 1

Interleukin 1β (IL-1β) mRNA expression in (A) post-infectious irritable bowel syndrome patients (INF-IBS) and in (B) post infection controls (INF-CON) (2% agarose gel stained with ethidium bromide; 30 polymerase chain reaction cycles). INF-IBS patients developed IBS after acute gastroenteritis and INF-CON patients returned to normal bowel habits after acute gastroenteritis

Figure 2

Changes in interleukin 1β (IL-1β) mRNA expression after infection in INF-IBS patients (who developed irritable bowel syndrome after acute gastroenteritis) and in INF-CON patients (who returned to normal bowel habits after acute gastroenteritis).

Figure 3

Changes in interleukin 1 receptor antagonist (IL-1ra) mRNA expression after infection in INF-IBS patients (who developed irritable bowel syndrome after acute gastroenteritis) and in INF-CON patients (who returned to normal bowel habits after acute gastroenteritis).