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. 2003 Mar;9(3):442-5.
doi: 10.3748/wjg.v9.i3.442.

Paclitaxel induces apoptosis in human gastric carcinoma cells

Hai-Bo Zhou  1 , Ju-Ren Zhu
Affiliations

Paclitaxel induces apoptosis in human gastric carcinoma cells

Hai-Bo Zhou et al. World J Gastroenterol. 2003 Mar.

Abstract

Aim: To investigate the apoptosis in gastric cancer cells induced by paclitaxel, and the relation between this apoptosis and expression of Bcl-2 and Bax.

Methods: In in vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission electron microscope and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of gastric cancer cell line SGC-7901 before and after the paclitaxel treatment. Immunohistochemical staining was used to detect the expression of apoptosis-regulated gene Bcl-2 and Bax.

Results: Paclitaxel inhibited the growth of gastric cancer cell line SGC-7901 in a dose-and time-dependent manner. Paclitaxel induced SGC-7901 cells to undergo apoptosis with typically apoptotic characteristics, including morphological changes of chromatin condensation, chromatin crescent formation, nucleus fragmentation and apoptotic body formation. Paclitaxel could reduce the expression of apoptosis-regulated gene Bcl-2, and improve the expression of apoptosis-regulated gene Bax.

Conclusion: Paclitaxel is able to induce the apoptosis in gastric cancer. This apoptosis may be mediated by down-expression of apoptosis-regulated gene Bcl-2 and up-expression of apoptosis-regulated gene Bax.

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Figures

Figure 1
Figure 1
Paclitaxel-induced apoptosis in SGC-7901 cells with Transmission Electron Microscope Apoptotic cell with chro-matin condensation, chromatin crescent formation, nucleus fragmentation ( × 4000).
Figure 2
Figure 2
Apoptotic cells induced by paclitaxel with TUNEL assay ( × 200)
See this image and copyright information in PMC

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