Alleviation of cyclophosphamide-induced urotoxicity by naturally occurring sulphur compounds

Abstract

Treatment of Swiss albino mice with naturally occurring sulphur compounds such as Diallyl sulphide (DAS) (500 microg/dose/animal, i.p) and Diallyl Disulphide (DADS) (250 microg/dose/animal, i.p) for five days along with cyclophosphamide (CTX) (1.5 mmol/kg.body.wt; i.p) reduced the CTX induced urotoxicity. Morphological analysis of the urinary bladders of the CTX treated group showed severe inflammation and dark colouration whereas the sulphur compounds treated group showed almost normal bladder morphology. Treatment with DAS and DADS was found to reduce the urine urea N2 level DAS (15.31+/-0.01 g/l), DADS (12.38+/-0.17 g/l) 4 hrs after CTX administration which was elevated in the CTX alone treated group (26.87+/-1.86 g/l). Urine protein level, which was enhanced drastically (8.66+/-0.47 g/l) after the CTX treatment, was significantly reduced (2.9+/-0.25 g/l) by the treatment with DADS. Similarly GSH content (which was drastically reduced by the CTX administration) in both bladder (0.87+/-0.1 nmol/mg protein) and liver (2.47+/-0.6 nmol/mg protein) was enhanced significantly (P<0.001) by the treatment with DAS and DADS both in bladder (DAS--1.56+/-0.17 nmol/mg protein; DADS--2.65+/-0.21 nmol/mg protein) and liver (DAS--5.30+/-0.07 nmol/mg protein; DADS--6.93+/-0.06 nmol/mg protein). Histopathological analysis of the bladder of the CTX alone treated group showed severe necrosis of the tissue whereas the sulphur compounds treated group showed normal bladder pathology.