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. 2003 Apr;87(4):409-10.
doi: 10.1136/bjo.87.4.409.

Confocal microscopy of Aspergillus fumigatus keratitis

A M Avunduk  1 , R W Beuerman, E D Varnell, H E Kaufman
Affiliations

Confocal microscopy of Aspergillus fumigatus keratitis

A M Avunduk et al. Br J Ophthalmol. 2003 Apr.

Abstract

Aim: To use a confocal microscope to characterise the treated and untreated courses of fungal keratitis.

Methods: In the first experiment, Aspergillus fumigatus stromal keratitis was produced in both eyes of seven New Zealand white rabbits. In the second experiment, keratitis was induced in right eyes of 20 rabbits. Group 1 rabbits were treated with topical fluconazole, group 2 rabbits received oral fluconazole, and group 3 rabbits were used as controls. The rabbits were examined with a slit lamp and confocal microscope 2, 6, 10, 14, and 20 days after inoculation. The corneal cultures were taken on days 2, 14, and 20 and biopsies were taken on days 2 and 22.

Results: On days 14 and 22 confocal microscopy was more sensitive than culture technique in both treated and untreated animals, since not all cases of fungal keratitis can be cultured.

Conclusion: This study indicates that confocal microscopy is a rapid and sensitive diagnostic tool for both the early diagnosis and non-invasive follow up of fungal keratitis

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Figures

Figure 1
Figure 1
Confocal microscopy, 2 days after Aspergillus fumigatus inoculation. Note hyphae (arrows), in disrupted superficial corneal stroma is parallel to corneal surface with branching at a 45° angle.
Figure 2
Figure 2
Confocal microscopy, 20 days after Aspergillus fumigatus inoculation of oral fluconazole treated rabbit cornea, shows small hyphal fragments (arrow).
See this image and copyright information in PMC

Comment in

References

    1. Cavanagh HD, Petroll WM, Alizadeh AH. Clinical and diagnostic use of in vivo confocal microscopy in patients with corneal disease. Ophthalmology 1993;100:1444–54. - PubMed
    1. Chew SJ, Beuerman RW, Assouline M, et al. Early diagnosis of infectious keratitis with in vivo real time confocal microscopy. CLAO J 1992;18:197–201. - PubMed
    1. Winchester K, Mathers WD, Sutphin JE. Diagnosis of Aspergillus keratitis in vivo with confocal microscopy. Cornea 1997;16:27–31. - PubMed
    1. Florakis GJ, Moazami G, Schubert H, et al. Scanning slit confocal microscopy of fungal keratitis. Arch Ophthalmol 1997;115:1461–63 - PubMed
    1. Liesegang TJ, Forster RK. Spectrum of microbial keratitis in South Florida. Am J Ophthalmol 1980;90:38–47. - PubMed