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. 2003 Apr;47(4):1297-300.
doi: 10.1128/AAC.47.4.1297-1300.2003.

Imipenem resistance in a Salmonella clinical strain due to plasmid-mediated class A carbapenemase KPC-2

Vivi Miriagou  1 , Leonidas S Tzouvelekis, Shannon Rossiter, Eva Tzelepi, Frederick J Angulo, Jean M Whichard
Affiliations

Imipenem resistance in a Salmonella clinical strain due to plasmid-mediated class A carbapenemase KPC-2

Vivi Miriagou et al. Antimicrob Agents Chemother. 2003 Apr.

Abstract

A Salmonella enterica serotype Cubana isolate exhibiting resistance to most beta-lactam antibiotics, including oxyimino-cephalosporins and imipenem, was isolated from a 4-year-old boy with gastroenteritis in Maryland. beta-Lactam resistance was mediated by a conjugative plasmid that encoded KPC-2, a class A carbapenemase previously found in a Klebsiella pneumoniae isolate from the Maryland area as well. Sequence analysis of the flanking regions indicated a potential association of bla(KPC-2) with mobile structures.

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Figures

FIG. 1.
FIG. 1.
(A) Isoelectric focusing of β-lactamase preparations from Salmonella serotype Cubana 4707, E. coli(pST4707), and E. coli(pST-H1) (lanes 2, 3, and 4, respectively). β-Lactamases with known pIs are in lane 1 (TEM-1, pI 5.4; PSE-3, pI 6.9; SHV-1, pI 7.6; SHV-5, pI 8.2). (B) Comparison of preparations from K. pneumoniae 1534 (lane 2) and Salmonella serotype Cubana 4707 (lane 3). β-Lactamases with known pIs are in lane 1 (TEM-12, pI 5.25; TEM-2, pI 5.6; SHV-3, pI 7.0; MIR-1, pI 8.4).
FIG. 2.
FIG. 2.
Schematic representation of the cloned HindIII fragment (GenBank accession number AF481906). Note that an additional HindIII site is located within the blaKPC-2 gene.
See this image and copyright information in PMC

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