Haematocrit: within-subject and seasonal variation

Abstract

This review was undertaken, concerning within-subject biological variation and seasonal variation of haematocrit in normal healthy adults and athletes, to find the limits for natural, intra-individual variation in haematocrit values. The terminology and calculations followed well defined theories, from the field of laboratory medicine, about biological variation. Based on results from 12 studies of 638 normal healthy adults, and which used a sampling interval of 1 day to 1-2 months, the coefficient of within-subject biological variation of haematocrit is 3%. The normal within-subject biological variation (3%) and analytical variation (3%) may explain a relative change of approximately 12% (95% level) [e.g. a change from 0.42-0.47] between two successive haematocrit values, measured with a time interval between 1 day and 1-2 months, in a normal healthy adult. Partly due to haemodilution in warm weather, haematocrit often has a seasonal variation in normal healthy adults; based on results from 18 studies of 24 793 participants, the population mean is approximately 3% lower in summer than winter. Population mean values that are 7% lower in summer than winter have been found in some studies, although no seasonal effect may also be seen, especially in temperate climates. If haematocrit values are sampled at yearly peak and trough time points, with intervals of up to 6 months, a 15% relative change (95% level) can be seen in a normal healthy adult; e.g. a change from 0.42-0.48. Published values for haematocrit in athletes are scarce. It is known that the haematocrit value is influenced by training, especially in the first weeks before a new steady-state is reached. Theoretically, the biological variation in athletes could therefore be greater than in normal individuals. Based on two references addressing the biological variation of haematocrit in athletes, however, the above results are also valid for athletes. Further studies, both in the short term and throughout the seasons, are recommended about the natural variation of haematocrit in athletes.