Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Mar;41(1):35-9.
doi: 10.3347/kjp.2003.41.1.35.

Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

Min-Ho Choi  1 , Il Chan Park, Shunyu Li, Sung-Tae Hong
Affiliations

Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA

Min-Ho Choi et al. Korean J Parasitol. 2003 Mar.

Abstract

Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Distribution of absorbances of various tested sera with crude antigen (•) and excretory-secretory antigen (▴) of Clonorchis sinensis. Ct, Control; Cs, Clonorchis sinensis; Ov, Opisthorchis viverrini; Pw, Paragonimus westermani; Sj, Schistosoma japonicum; Fh, Fasciola hepatica.
See this image and copyright information in PMC

References

    1. Chen CY, Hsieh WC, Shih HH, Chen SN. Immunodiagnosis of clonorchiasis by enzyme-linked immunosorbent assay. Southeast Asian J Trop Med Public Health. 1988;19:117–121. - PubMed
    1. Cho SY, Kong Y, Yun DH, et al. Persisting antibody reaction in paragonimiasis after praziquantel treatment is elicited mainly by egg antigens. Korean J Parasitol. 2000;38:75–84. - PMC - PubMed
    1. Chung YB, Chung BS, Choi MH, Chai JY, Hong ST. Partial characterization of a 17 kDa protein of Clonorchis sinensis. Korean J Parasitol. 2000;38:95–97. - PMC - PubMed
    1. Chung YB, Lee M, Yang HJ, et al. Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis. Korean J Parasitol. 2002;40:83–88. - PMC - PubMed
    1. Hong ST. Changes of anti-Clonorchis sinensis IgG antibody in serum after praziquantel treatment in human clonorchiasis. Korean J Parasitol. 1988;16:1–8. - PubMed

MeSH terms

Substances

LinkOut - more resources